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Transformation Protocols

The protocols detailed here use Agrobacterium-mediated transformation, however we have also included a wheat biolistic protocol.

The protocol for each main crop below gives step by step instructions for the transformation of that crop together with media required and appropriate references. The accompanying barley and brassica files are diagrammatic representations of the methodology. NEW Wheat Agrobacterium-mediated transformation protocol NEW

Barley Protocol 87.8KB Poster of Barley Protocol 1.08MB NEW Barley Tiller Selection Photos 132KB
Brassica Protocol 128KB Diagram of Brassica Protocol 52.2KB Brassica Transformation Photos 164KB
NEW Wheat Agrobacterium Protocol Wu et al. (2003) paper 391KB Wheat Biolistic Protocol 108KB

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It is important to always carry out laboratory procedures in compliance with local health and safety rules.

If you require further health and safety information please click here.

Updated 25th August 2005 by enquiries@bract.org
The John Innes Centre and Rothamsted Research provides no express or implied warranties of any kind, including but not limited to those of merchantability, fitness for a particular purpose and non-infringement of the proprietary rights, such as copyrights, patents and trade secrets, of third parties. The John Innes Centre and Rothamsted Research makes every effort to ensure the quality and accuracy of the material on the BRACT web pages. However, it accepts no liability for the contents of the John Innes Centre and Rothamsted Research BRACT web pages, including any third party material.