A summary of BRACT resources and services are given below.
Transformation Resources: Access to Protocols/Germplasm/Training/Constructs
- Easy to follow transformation Protocols: For Wheat, Barley and Brassica
- Germplasm (of readily transformable genotypes/ together with advice on screening methods to identify suitable candidate genotypes for transformation)
Starter packs of seed, of our recommended lines, are available on request. A fee to cover the cost of production will be charged. Recommended genotypes for routine studies are:
Brassica: B. oleracea spring genotype AG DH1012 (we also support transformation in B. napus – contact us for further details of recommended genotypes)
Barley: the spring variety Golden Promise
Wheat: the spring variety Fielder
- Hosted training programmes: the John Innes Centre offers hosted 1-2 week training programmes for researchers wanting to come and gain hands-on experience for both Brassica and barley transformation. We can also accommodate a number of self funding visiting workers, and provide access to research hotel facilities for researchers wishing to stay for longer periods to carry out their own transformation work.
- Constructs: access to a range of tried and tested pBRACT constructs, developed for each of the main crops are available together with a complete construct build service )
- A full range of transformation services (from supply of primary transgenics, through to seed of subsequent generations, molecular analysis and basic phenotyping).
- Help and advice with grant applications and project planning. Where BRACT resources are to be incorporated in proposals - the BRACT team can help with grant applications by providing summaries/ cost break downs/ evaluation of timescale/ objective feasibility and provision of letters of support.