Publications

Harwood WA, (2011) Advances and remaining challenges in the transformation of barley and wheat. J Exp Bot. doi:10.1093/jxb/err380.

Cullen D, Harwood WA, Smedley MA, Davies H, Taylor M. (2011) Comparison of DNA walking methods for isolation of transgene-flanking regions in GM potato. Molecular Biotechnology, 49: 19-31.

Stanley D., Rejzek M., Naested H., Smedley M., Otero S., Fahy B., Thorpe F., Nash R. J., Harwood W., Svensson B., Denyer K., Field R. A., Smith A. M. (2011) The role of {alpha}-glucosidase in germinating barley grains Plant Physiology 155: 932-943

Sparrow P. A. C., Goldsack C., Ostergaard L.(2011) Transformation technology in the Brassicaceae Genetics and Genomics of the Brassicaceae Springer 505-526

Sparrow P. A. C., Smedley M., Harwood W.(2011) Brassica transformation resources Aspects of Biology GM From Basic Research to Application 32-36

L Xia, Y Ma, Yi He and HD Jones (2011) GM wheat development in China: current status and challenges to commercialization. J. Exp. Bot. On-line 15th Dec 2011 doi:10.1093/jxb/err342

X Yu, HD. Jones, Y Ma, G Wang, Z Xu, B Zhang, Y Zhang, G Ren, JA. Pickett & L Xia (2011) (E)-β-Farnesene synthase genes affect aphid (Myzus persicae) infestation in tobacco (Nicotiana tabacum). Functional & Integrative Genomics.DOI 10.1007/s10142-011-0244-1

YW Sun, HD. Jones, Y Yang, S Dreisigacker, SM Li XM Chen, PR. Shewry and LQ. Xia (2011) Haplotype analysis of Viviparous-1 gene in CIMMYT elite bread wheat germplasm. Euphytica (In Press).

J. Freeman, C.A. Sparks, J. West, P. R. Shewry & H.D. Jones (2011). Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat. Plant Biotechnology Journal  9: 788–796

Sparrow P. A.(2010) GM risk assessment. Molecular Biotechnology 44 267-75

C Nemeth, J Freeman, HD Jones, C Sparks, TK Pellny, D Wilkinson, J Dunwell, AAM Andersson, P Åman, F Guillon, L Saulnier, RAC Mitchell and PR Shewry (2010). Down-regulation of the CSLF6 gene results in decreased (1,3;1,4)-β-D-glucan in endosperm of wheat. Plant Physiology. 152: 1209-1218.

F Sestili, M Janni, A Doherty, E Botticella, R D’Ovidio, S Masci, HD Jones, D Lafiandra (2010).  Increasing the amylose content of durum wheat through silencing of the SBEIIa genes. BMC Plant Biology, 10:144.

Y. He, HD. Jones, S. Chen, X.M. Chen, DW. Wang, KX. Li, DS. Wang and LQ. Xia (2010). Agrobacterium-mediated transformation of durum wheat (Triticum turgidum L. var. durum cv Stewart) with improved efficiency. Journal Experimental Botany, 61: 1567 - 1581.

Bartlett J G, Snape J W, Harwood W A (2009) Intron-mediated enhancement as a method for increasing transgene expression levels in barley. Plant Biotechnology Journal, 7: 856-866.

Harwood W A, Bartlett J, Alves S, Perry M, Smedley M, Leyland N, Snape JW (2009) Barley transformation using Agrobacterium-mediated techniques. In: Huw D Jones and Peter R Shewry (eds), Methods in Molecular Biology, Transgenic Wheat, Barley and Oats, Vol 478, Chapter 9, pp137-147.

Harwood W A, Smedley M (2009) Barley transformation using biolistic techniques. In: Huw D Jones and Peter R Shewry (eds), Methods in Molecular Biology, Transgenic Wheat, Barley and Oats, Vol 478, Chapter 8, pp125-136.

Xia L. Q., Yang Y., Ma Y. Z., Chen X. M., He Z. H., Roder M. S., Jones H. D. and Shewry P. R. (2009). What can the Viviparous-1 gene tell us about wheat pre-harvest sprouting? Euphytica 168:385–394

Jones HD, Sparks CA & Shewry PR (2009) Transgenic manipulation of wheat quality. In: Wheat; Chemistry and Technology 4th Ed. Eds. K Khan & PR Shewry. AACC International MN USA.

Blechl AE and Jones HD (2009) Transgenic Applications in Wheat Improvement. In Wheat: Science and Trade, Ed. Brett F. Carver, Blackwell Publishing.

HD Jones and PR Shewry (2009) Editors and authors, Methods in Biotechnology. Transgenic Wheat, Barley and Oats: Production and Characterisation. Series Ed. J Walker. Humana Press Totowa NJ.

Jones Huw D and Sparks CA (2009) Stable Transformation of Plants. In: Plant Genomics, Methods in Molecular Biology. Eds D Somers, P Langridge and JP Gustafson. Humana Press Totowa, NJ.

Jones Huw D and Sparks CA (2009) Transient Transformation of Plants. In: Plant Genomics, Methods in Molecular Biology. Eds D Somers, P Langridge and JP Gustafson. Humana Press Totowa, NJ.

Bartlett  J G, Alves S C, Smedley M, Snape J W, Harwood W A (2008) High-throughput Agrobacterium-mediated barley transformation. Plant Methods, 4: 22.

Sparrow P.A.C.  Book Chapter: GM Risk Assessment. Transgenic Wheat, Barley and Oats: Production and Characterisation (2008). Methods in Biotechnology. 2008:478:315-330. Editors Peter Shewry and Hue Jones.

LF Primavesi, H Wu, EA Mudd, A Day and HD Jones  (2008) Visualisation of plastids in endosperm, pollen and roots of transgenic wheat expressing modified GFP fused to transit peptides from wheat SSU RubisCO, rice FtsZ and maize ferredoxin III proteins. Transgenic Research 17(4): 529-543.

Van Herpen, T., Riley, M., Sparks, C., Jones, H.D., Gritsch, C., Dekking, E.H., Hamer, R.J., Bosch, D., Salentijn, E.M.J., Smulders, M.J.M., Shewry, P.R. and Gilissen, L. (2008) Detailed analysis of the expression of an alpha-gliadin promoter and the deposition of alpha-gliadin protein during wheat grain development. Annals of Botany, 102, 331-342.

H Wu, A Doherty and H D Jones (2008). Efficient and rapid Agrobacterium-mediated genetic transformation of durum wheat (T. turgidum L. var. durum) using additional virulence genes. Transgenic Research 17(3): 425-436.

M. Rakszegi, G. Pastori, H.D. Jones, F. Békés, B. Butow, L. Láng, Z. Bedő and P.R. Shewry (2008). Technological quality of field grown transgenic lines of commercial wheat cultivars expressing the 1Ax1 HMW glutenin subunit gene. Journal of Cereal Science. 47(2): 310-321.

PR Shewry, HD Jones & NG Halford (2008). Plant Biotechnology: Transgenic Crops. In: Advances in Biochemical Engineering and Biotechnology. Vol.111. Stahl U; Donalies UEB; Nevoigt E (Eds.) 2008, XII, 269p. Springer.

Jones HD, Freeman JA and Sparks CA (2008). A Transgenic approach to understanding gene expression in cereals. In: Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. (Options méditerranéennes série A. Séminaires Méditerranéens, N° 81) Eds.: Molina Cano J.L., Christou P., Graner A., Hammer K., Jouve N., Keller B., Lasa J.M., Powell W., Royo C., Shewry P., Stanca A.M. EUCARPIA/CIHEAM. ISBN : 2-85352-404-3

Sparrow P.A.C, Irwin J.A. , Goldsack C., Ostergaard L(2007) Brassica transformation: commercial application and powerful research tool Transgenic Plant Journal 1 330-339

Appleford NEJ, Wilkinson MD, Ma Q, Evans DJ, Stone MC, Pearce SP, Powers SJ, Thomas SG, Jones HD, Phillips AL, Hedden P and Lenton JR (2007) Decreased shoot stature and grain α-amylase activity following ectopic expression of a GA2-oxidase gene in transgenic wheat. Journal of Experimental Botany 58(12): 3213-3226.

GM Pastori, A Huttly, J West, C Sparks, A Pieters, CM. Luna, HD. Jones and CH. Foyer (2007). The maize Ac/Ds system is functional in hexaploid wheat through successive generations. Functional Plant Biology 34:835-843.

Y Yang, YZ Ma, ZS Xu,  XM Chen, ZH He, Z Yu, M Roder, M Wilkinson, HD Jones, PR Shewry and LQ Xia (2007). Isolation and Expression of Novel Viviparous-1 Genes in Common Wheat (Triticum aestivum L.).  Journal of Experimental Botany 58(11): 2863-2871.

PR Wiley, P Tosi, A Evrard, A Lovegrove, HD Jones and PR Shewry (2007). Promoter Analysis and Immunolocalisation Show That Puroindoline Genes are Exclusively Expressed in Starchy Endosperm Cells of Wheat Grain. Plant Molecular Biology, 64: 125-136.

Zhang JU, Li K, Yang G, Luo L, Jones HD, Shewry PR, He G. (2007) Combined Expression of Gluten Strength and Grain Hardness Genes by Crossing Transgenic Plants with Elite Wheat Varieties. In Gluten Proteins 2006, Eds. GL Lookhart & PKW Ng. AACC International Inc.

Shewry PR & Jones HD (2007). Developing allergen-free foods by genetic manipulation. In Managing Allergens in Food, Eds. C Mills, H Wichers & K Hoffmann-Sommergruber. CRC Press.  Woodhead publishing Ltd. Cambridge UK.

Sparrow P. A. C., Snape J. W., Dale P. J., Irwin J. A.(2006) The rapid identification of B.napus genotypes, for high-throughput transformation, using phenotypic tissue culture markers Acta Horticulturae 706 239-247

Sparrow P. A. C., Irwin J. A.(2006) The use of phenotypic markers to identify brassica genotypes for routine high throughput agrobacterium mediated transformation Acta Horticulturae: IV International Symposium on Brassicas and XIV Crucifer Genetics Workshop 706 239-247

Sparrow P. A. C., Irwin J. A. (2006) Brassica oleracea Methods in Molecular Biology Series 2nd ed. Vol.1: Agrobacterium Protocols (Editors: Wang, K.) chapter 35 417-427

Travella, S, Ross S M, Harden J,  Everett C,  Snape J W, Harwood W A (2005) A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques. Plant Cell Reports 23: 780-789.

H Wu, CA Sparks & HD Jones (2006). Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium-mediated transformation. Molecular Breeding, 18:195-208.

Peter R Shewry, Stephen Powers, J Michael Field, Roger J Fido, Huw D Jones, Gillian M Arnold, Jevon West, Paul A Lazzeri, Pilar Barcelo, Francisco Barro, Arthur S Tatham, Frank Bekes, Barbara Butow and Helen Darlington (2006). Comparative Field Performance over Three Years and Two Sites of Transgenic Wheat Lines Expressing HMW Subunit Transgene. Theor. Appl. Genet. 113:128-136.

Jones HD (2006). Advances in Transformation Technologies. In Plant Biotechnology, Current and Future Applications of Genetically Modified Crops. Ed. Nigel Halford. John Wiley & Sons, Ltd. Chichester, UK. ISBN 0470021810.

PR Shewry, HD Jones & NG Halford (2006). Plant Biotechnology; Transgenic Crops. In Advances in Biochemical Engineering/Biotechnology, Special Volume: Food Biotechnology Eds Stahl & Donalies, Springer Press.

Huw D Jones, Angela Doherty and Huixia Wu (2005). Review of Methodologies and a Protocol for the Agrobacterium-mediated transformation of wheat. Plant Methods 1:5.

Shewry PR and Jones HD. (2005). Transgenic wheat: where do we stand after the first 12 years? Annals of Applied Biology 147 (1), 1-14.

Guang Y He; Huw D Jones; Renato D'Ovidio; Stefania Masci; Mingjie Chen; Jevon West; Barbara Butow; Olin D Anderson; Paul Lazzeri; Roger Fido; Peter Shewry Expression of an extended HMW subunit in transgenic wheat and the effect on dough mixing properties (2005).  Journal of Cereal Science. 42:225-231.

Jonathan R. Howarth, Jean N. Jacquet, Angela Doherty, Huw D. Jones  and Martin E. Cannell (2005). Molecular genetic analysis of silencing in two lines of Triticum aestivum transformed with pAHC25. Annals of Applied Biology 146:311-320.

Jones Huw D (2005). Wheat transformation: Current technology and applications to grain development and composition. Journal of Cereal Science. Vol 41 (2): 137-147

Chrimes D, Rogers HJ, Frances D, Jones HD and Ainsworth C (2005). Expression of fission yeast cdc25 driven by the wheat ADP-glucose pyrophosphorylase large subunit promoter perturbs both pollen and ovule development in transgenic wheat. NewPhytologist, Vol 166 (1): 185-192.

N-N Shi, He G-Y, Li KX, Jones HD & Shewry PR (2005). Transformation and Expression of High Molecular Weight Glutenin Subunit Genes in Common Wheat (Triticum aestivum L.) varieties. Scientia Agricultura Sinica 38(5): 874-881.

J Goodwin, G Pastori, M Davey & H D Jones (2005).  Selectable Markers: antibiotic and herbicide resistance, pp 191-203. In Transgenic Plants: Methods and Protocols. Ed. L Pena. Humana Press.

Bourdon V, Wickham A, Lonsdale D, Harwood W A, (2004) Additional introns inserted within the luciferase reporter gene stabilize transgene expression in wheat. Plant Science 167: 1143-1149.

Salvo G H, Travella S, Bilham L J, Harwood W A, Snape J W (2004) The distribution of transgene insertion sites in barley determined by physical and genetic mapping. Genetics 167: 1371-1379.

Harwood W A, Bilham L J, Travella S, Salvo-Garrido H, Snape J W (2004) Fluorescence in situ hybridization to localise transgenes in plant chromosomes. Methods in Molecular Biology: Transgenic Plants (Ed. L Pena) Vol. 286: 327-340.

Sparrow P. A. C., Townsend T. M., Arthur A. E., Dale P. J., Irwin J. A.(2004) Genetic analysis of Agrobacterium tumefaciens susceptibility in Brassica oleracea Theoretical and Applied Genetics 108 644-650

Sparrow P. A. C., Townsend T. M., Morgan C. L., Dale P. J., Arthur A. E., Irwin J. A.(2004) Genetic analysis of in vitro shoot regeneration from cotyledonary petioles of Brassica oleracea Theoretical and Applied Genetics 108 1249-1255

Sparrow P. A. C., Dale P. J., Irwin J. A.(2004) The use of phenotypic markers to identify Brassica oleracea genotypes for routine high-throughput Agrobacterium-mediated transformation. Plant Cell Reports 23 64-70