pBRACT Introduction

• The pBRACT vectors have been designed for crop transformation, and specifically for expression studies in wheat, barley and Brassica. pBRACT constructs are also available for pea/Arabidopsis transformation.
• The pBRACT vectors are based on pGreen, which is a small, versatile vector designed for easy manipulation in E.coli with a high copy number.
  To enable the small size of pGreen, the pSa origin of replication required for replication in Agrobacterium, is separated into its’ two distinct functions. The replication origin (ori) is present on pGreen, and the trans-acting replicase gene (RepA) is present on an additional vector, named pSoup. Both vectors are required in Agrobacterium for pGreen to replicate.

• The pBRACT vectors have been designed as Gateway® destination vectors. They therefore use Gateway® cloning as the method for gene introduction, which is simple and reliable.
  Gateway® cloning is based on the site-specific recombination reactions that occur when bacteriophage lambda enters the lytic and/or lysogenic pathway when a host cell is invaded. To utilise the Gateway® recombination reactions your gene of interest first needs to be cloned into a pENTRY vector. Your gene can then be introduced into any one of the pBRACT destination vectors using a one tube overnight reaction.